PENGARUH PENAMBAHAN NATRIUM HIPOKLORIT (NaOCl) TERHADAP MIKROBA PADA AIR LIMBAH DENGAN METODE MEMBRANE FILTER

Pevi Riani, Hijratul Hidayana

Abstract

This research was conducted to determine the effect of adding sodium hypochlorite (NaOCl) on the number of E. coli and Total Coliform bacterial colonies in industrial wastewater using membrane filters. The prepared wastewater is added with NaOCl with concentration variations 0 ; 0.5 ; 1 ; 1.5 ; 2 ; 3 ; 4 ; 5 ppm. The vacuum pump and filter membrane are placed in laminar air flow and the filtration process is carried out. The filtered membrane paper was placed into a petri dish containing CCA agar media and incubated for 24-48 hours at a temperature of 35oC with the petri dish upside down. Reddish pink colonies indicate colonies of Coliform bacteria, while dark blue or violet colonies are colonies of Escherichia coli bacteria. The results showed that the number of E. coli bacteria at NaOCl concentrations of 0 ppm to 5 ppm was 0 cfu/100 mL and Total Coliform was obtained at NaOCl concentrations of 0 ; 0,5 ; 1 ; 1,5 ; 2 ; 3 ; 4 dan 5 ppm is cannot be Calculated, 96 ; 74 ; 53 ; 31 ; 19 ; 3 and 0 cfu/100 mL.

Keywords

Escherichia coli, Coliform, Membrane Filter, Wastewater

Full Text:

PDF

References

Alaerts, G., dan Sumestri S., 1987. Metode Penelitian Air. Usaha Nasional. Surabaya.

Alcamo, I. E. 1996. Laboratory Fundamentals of Microbiology. Farmingdale: Addison Wesley Publishing Company.

Arya, S.P. 2004. Pengantar Teknik Lingkungan. Bandung. Penerbit ITB

Arya Wardana, Wisnu. 2001. Dampak Pencemaran Lingkungan. Penerbit Andi. Yogyakarta.

Bhatti, A. A., Usman, M., Kandi, V., & Mustafa, H. (2017). Microbial pathogenesis of Helicobacter pylori and strategies for combating drug resistance. Microbial pathogenesis, 111, 156-163.

Effendi. 2003. Telaah Kualitas Air: Pengelolaan Sumber Daya dan Lingkungan Perairan. Yogyakarta : Kanisius

Fardiaz, S. 1993. Analisis Mikrobiologi Pangan. PT. Raja Grafindo Persada. Jakarta.

Fuadi, Azhar. 2012. Pengaruh Residual Klorin Terhadap Kualitas Mikrobiologi Pada Jaringan Distribusi Air Bersih. Universitas Indonesia. Depok

Irianto, K., 2006. Mikrobiologi: Menguak Dunia Mikroorganisme. Bandung: CV. Yrama Widya.

Jiwintarum, Y., Agrijanti, & Septiana, B. L. (2017). Most Probable Number (MPN) Coliform Dengan Variasi Volume Media Lactose Broth Single Strength (LBSS) dan Lactose Broth Double Strength (LBDS). Jurnal Kesehatan Prima, 11(1), 1.

Kementerian Lingkungan Hidup dan Kehutanan. (2016). Peraturan Menteri LHK No.68 th 2016 tentang Baku Mutu Air Limbah Domestik. Kementerian Lingkungan Hidup Dan Kehutanan, 68, 1–13.

Murtadho, D. dan G. Said. 1988. Penaganan dan Pemanfaatan Limbah Padat. Penerbit Mediyatama Sarana Perkasa, Jakarta.

Kristanto, D. 2004. Manajemen Lingkungan. Jakarta : PT. Grasindo

Nugrayanti, M. S., Dermawan, D., & Dewi, T. U. (2018). Pengaruh pemberian dosis trichloroisocyanuric acid (tcca) pada bak desinfeksi terhadap penurunan kandungan escherichia coli di RSUD Dr. R. Koesma Tuban. Conference Proceeding on Waste Treatment Technology, 1(1), 129–134.

Peraturan Menteri Kesehatan Nomor 32 Tahun 2017. Tentang Standar Baku Mutu Kesehatan Lingkungan dan Persyaratan Kesehatan Air untuk Keperluan Higiene Sanitasi. Kementerian Kesehatan, hal 11

Prescott, L. M., Harley, J. P., & Klein, D. A. (2016). Microbiology (9th ed.). McGraw-Hill Education.

DOI: http://dx.doi.org/10.52759/sainti.v20i2.238

Refbacks

  • There are currently no refbacks.